{"id":8755,"date":"2025-01-01T16:52:47","date_gmt":"2025-01-01T14:52:47","guid":{"rendered":"https:\/\/medilab.km.ua\/?p=8755"},"modified":"2026-04-20T18:54:44","modified_gmt":"2026-04-20T15:54:44","slug":"crispr-diagnostics-revolutionizes-the-accuracy-and-speed-of-disease-detection","status":"publish","type":"post","link":"https:\/\/medilab.km.ua\/en\/crispr-diagnostics-revolutionizes-the-accuracy-and-speed-of-disease-detection\/","title":{"rendered":"CRISPR diagnostics: a revolution in the accuracy and speed of disease detection"},"content":{"rendered":"<div class=\"kk-star-ratings kksr-auto kksr-align-left kksr-valign-top\"\n    data-payload='{&quot;align&quot;:&quot;left&quot;,&quot;id&quot;:&quot;8755&quot;,&quot;slug&quot;:&quot;default&quot;,&quot;valign&quot;:&quot;top&quot;,&quot;ignore&quot;:&quot;&quot;,&quot;reference&quot;:&quot;auto&quot;,&quot;class&quot;:&quot;&quot;,&quot;count&quot;:&quot;900&quot;,&quot;legendonly&quot;:&quot;&quot;,&quot;readonly&quot;:&quot;&quot;,&quot;score&quot;:&quot;4.8&quot;,&quot;starsonly&quot;:&quot;&quot;,&quot;best&quot;:&quot;5&quot;,&quot;gap&quot;:&quot;5&quot;,&quot;greet&quot;:&quot;Rate this post&quot;,&quot;legend&quot;:&quot;4.8\\\/5 - (900 votes)&quot;,&quot;size&quot;:&quot;24&quot;,&quot;title&quot;:&quot;CRISPR-\u0434\u0456\u0430\u0433\u043d\u043e\u0441\u0442\u0438\u043a\u0430: \u0440\u0435\u0432\u043e\u043b\u044e\u0446\u0456\u044f \u0432 \u0442\u043e\u0447\u043d\u043e\u0441\u0442\u0456 \u0442\u0430 \u0448\u0432\u0438\u0434\u043a\u043e\u0441\u0442\u0456 \u0432\u0438\u044f\u0432\u043b\u0435\u043d\u043d\u044f \u0437\u0430\u0445\u0432\u043e\u0440\u044e\u0432\u0430\u043d\u044c&quot;,&quot;width&quot;:&quot;136.7&quot;,&quot;_legend&quot;:&quot;{score}\\\/{best} - ({count} {votes})&quot;,&quot;font_factor&quot;:&quot;1.25&quot;}'>\n            \n<div class=\"kksr-stars\">\n    \n<div class=\"kksr-stars-inactive\">\n            <div class=\"kksr-star\" data-star=\"1\" style=\"padding-right: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" data-star=\"2\" style=\"padding-right: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" data-star=\"3\" style=\"padding-right: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" data-star=\"4\" style=\"padding-right: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" data-star=\"5\" style=\"padding-right: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n    <\/div>\n    \n<div class=\"kksr-stars-active\" style=\"width: 136.7px;\">\n            <div class=\"kksr-star\" style=\"padding-right: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" style=\"padding-right: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" style=\"padding-right: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" style=\"padding-right: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" style=\"padding-right: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n    <\/div>\n<\/div>\n                \n\n<div class=\"kksr-legend\" style=\"font-size: 19.2px;\">\n            4.8\/5 - (900 votes)    <\/div>\n    <\/div>\n<p class=\"\" data-start=\"81\" data-end=\"493\">Until recently, CRISPR was associated exclusively with genome editing. However, today this tool is used not only for changing DNA, but also for extremely accurate, fast and affordable diagnosis of diseases. CRISPR diagnostics is a new stage in the development of molecular medicine, which promises to significantly change the approach to detecting viruses, bacterial infections, cancer mutations and even genetic diseases.<\/p>\n<blockquote data-start=\"495\" data-end=\"700\">\n<p class=\"\" data-start=\"497\" data-end=\"700\">\u00abCRISPR is like the Swiss Army knife of molecular biology. It not only edits, but can also instantly detect changes in the DNA or RNA of pathogens,\u00bb says molecular biologist Richard Dylan of the UCL Genetics Institute.<\/p>\n<\/blockquote>\n<h3 class=\"\" data-start=\"702\" data-end=\"732\"><strong>What is CRISPR diagnostics?<\/strong><\/h3>\n<p class=\"\" data-start=\"734\" data-end=\"1056\">The CRISPR\/Cas system is a bacterial \u00abimmunity\u00bb that scientists have adapted to work with genetic material. In diagnostics, CRISPR is used not to change DNA, but to recognize it. Thanks to the Cas12 or Cas13 proteins, which are activated when a target sequence is detected, it is possible to \u00absee\u00bb the presence of a pathogen or mutation.<\/p>\n<p class=\"\" data-start=\"1058\" data-end=\"1075\">Basic principles:<\/p>\n<ul data-start=\"1077\" data-end=\"1247\">\n<li class=\"\" data-start=\"1077\" data-end=\"1129\">\n<p class=\"\" data-start=\"1079\" data-end=\"1129\">determination of a specific DNA or RNA sequence<\/p>\n<\/li>\n<li class=\"\" data-start=\"1130\" data-end=\"1181\">\n<p class=\"\" data-start=\"1132\" data-end=\"1181\">instant response of the cleaver protein (Cas) to the target<\/p>\n<\/li>\n<li class=\"\" data-start=\"1182\" data-end=\"1247\">\n<p class=\"\" data-start=\"1184\" data-end=\"1247\">visualization of the result through fluorescence or color change<\/p>\n<\/li>\n<\/ul>\n<p><img decoding=\"async\" class=\"alignnone size-full wp-image-8757\" src=\"https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/03\/doctor-his-cabinet-looking-braind-trying-put-diagnosis-doctor-giving-his-expertise.jpg\" alt=\"Device for CRISPR analysis in the field\" width=\"1200\" height=\"800\" srcset=\"https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/03\/doctor-his-cabinet-looking-braind-trying-put-diagnosis-doctor-giving-his-expertise.jpg 1200w, https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/03\/doctor-his-cabinet-looking-braind-trying-put-diagnosis-doctor-giving-his-expertise-150x100.jpg 150w, https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/03\/doctor-his-cabinet-looking-braind-trying-put-diagnosis-doctor-giving-his-expertise-600x400.jpg 600w, https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/03\/doctor-his-cabinet-looking-braind-trying-put-diagnosis-doctor-giving-his-expertise-300x200.jpg 300w, https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/03\/doctor-his-cabinet-looking-braind-trying-put-diagnosis-doctor-giving-his-expertise-1024x683.jpg 1024w, https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/03\/doctor-his-cabinet-looking-braind-trying-put-diagnosis-doctor-giving-his-expertise-768x512.jpg 768w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/><\/p>\n<h3 class=\"\" data-start=\"1249\" data-end=\"1279\"><strong>What are the advantages of technology?<\/strong><\/h3>\n<p class=\"\" data-start=\"1281\" data-end=\"1397\"><strong data-start=\"1281\" data-end=\"1303\">1. High accuracy<\/strong><br data-start=\"1303\" data-end=\"1306\" \/>CRISPR looks for a specific genetic target, which helps avoid false positives.<\/p>\n<p class=\"\" data-start=\"1399\" data-end=\"1489\"><strong data-start=\"1399\" data-end=\"1415\">2. Speed<\/strong><br data-start=\"1415\" data-end=\"1418\" \/>Most tests take <strong data-start=\"1444\" data-end=\"1464\">less than an hour<\/strong>, and some - 20\u201330 minutes.<\/p>\n<p class=\"\" data-start=\"1491\" data-end=\"1618\"><strong data-start=\"1491\" data-end=\"1516\">3. Ease of implementation<\/strong><br data-start=\"1516\" data-end=\"1519\" \/>The methods can be adapted for portable devices, making testing possible in the field.<\/p>\n<p class=\"\" data-start=\"1620\" data-end=\"1761\"><strong data-start=\"1620\" data-end=\"1642\">4. Versatility<\/strong><br data-start=\"1642\" data-end=\"1645\" \/>CRISPR diagnostics applied to viruses (<a href=\"https:\/\/medilab.km.ua\/en\/price\/?3325_search_1=SARS-CoV-2&amp;3325_device=laptop&amp;3325_orderby=relevance&amp;3325_paged=1&amp;3325_filtered=true\">SARS-CoV-2<\/a>, HIV, flu), bacteria, cancer markers, hereditary mutations.<\/p>\n<blockquote data-start=\"1763\" data-end=\"1978\">\n<p class=\"\" data-start=\"1765\" data-end=\"1978\">\u00abCRISPR tests could be as simple as a pregnancy test \u2014 and just as affordable,\u00bb says Dr. Sarah Chang of the Broad Institute. \u00abWe\u2019re moving towards an era of rapid diagnostics right in the pharmacy or at home.\u00bb.<\/p>\n<\/blockquote>\n<h3 class=\"\" data-start=\"1980\" data-end=\"2008\"><strong>How it works in practice<\/strong><\/h3>\n<ol data-start=\"2010\" data-end=\"2314\">\n<li class=\"\" data-start=\"2010\" data-end=\"2079\">\n<p class=\"\" data-start=\"2013\" data-end=\"2079\">The sample (saliva, blood, urine, swab) is placed in the reaction mixture.<\/p>\n<\/li>\n<li class=\"\" data-start=\"2080\" data-end=\"2155\">\n<p class=\"\" data-start=\"2083\" data-end=\"2155\">The Cas protein, with a guide (RNA probe), searches for a target genetic sequence.<\/p>\n<\/li>\n<li class=\"\" data-start=\"2156\" data-end=\"2224\">\n<p class=\"\" data-start=\"2159\" data-end=\"2224\">When it is detected, Cas is activated and cuts a special marker.<\/p>\n<\/li>\n<li class=\"\" data-start=\"2225\" data-end=\"2314\">\n<p class=\"\" data-start=\"2228\" data-end=\"2314\">The result appears as a color change or glow in the test, which looks like a strip.<\/p>\n<\/li>\n<\/ol>\n<h3 class=\"\" data-start=\"2316\" data-end=\"2351\"><strong>Application of CRISPR diagnostics<\/strong><\/h3>\n<div class=\"overflow-x-auto contain-inline-size\">\n<table data-start=\"2353\" data-end=\"2918\">\n<thead data-start=\"2353\" data-end=\"2431\">\n<tr data-start=\"2353\" data-end=\"2431\">\n<th data-start=\"2353\" data-end=\"2380\">Region<\/th>\n<th data-start=\"2380\" data-end=\"2431\">Application examples<\/th>\n<\/tr>\n<\/thead>\n<tbody data-start=\"2512\" data-end=\"2918\">\n<tr data-start=\"2512\" data-end=\"2591\">\n<td>Infectious diseases<\/td>\n<td>SARS-CoV-2, tuberculosis, HIV, hepatitis<\/td>\n<\/tr>\n<tr data-start=\"2592\" data-end=\"2671\">\n<td>Oncology<\/td>\n<td>Detection of KRAS, BRAF, EGFR mutations<\/td>\n<\/tr>\n<tr data-start=\"2672\" data-end=\"2760\">\n<td>Genetic diseases<\/td>\n<td>Beta-thalassemia, cystic fibrosis, hereditary disorder syndromes<\/td>\n<\/tr>\n<tr data-start=\"2761\" data-end=\"2839\">\n<td>Veterinary medicine<\/td>\n<td>Animal diagnostics in the field<\/td>\n<\/tr>\n<tr data-start=\"2840\" data-end=\"2918\">\n<td>Agrobiotechnologies<\/td>\n<td>Checking for GMOs, phytopathogens in plants<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<h3 class=\"\" data-start=\"2920\" data-end=\"2950\"><strong>Example: CRISPR and COVID-19<\/strong><\/h3>\n<p class=\"\" data-start=\"2952\" data-end=\"3254\">During the COVID-19 pandemic, several CRISPR tests (e.g. SHERLOCK, DETECTR) were developed that detected the virus with an accuracy of more than 95%, without the need for complex laboratory equipment. The test gave a result in 30 minutes, and only biomaterial and a simple reaction tube were required for its execution.<\/p>\n<h3 class=\"\" data-start=\"3256\" data-end=\"3287\"><strong>The future of CRISPR diagnostics<\/strong><\/h3>\n<p class=\"\" data-start=\"3289\" data-end=\"3308\">Among the prospects:<\/p>\n<ul data-start=\"3309\" data-end=\"3571\">\n<li class=\"\" data-start=\"3309\" data-end=\"3378\">\n<p class=\"\" data-start=\"3311\" data-end=\"3378\">development of portable devices for daily health monitoring<\/p>\n<\/li>\n<li class=\"\" data-start=\"3379\" data-end=\"3436\">\n<p class=\"\" data-start=\"3381\" data-end=\"3436\">personalized cancer marker tests, right in the clinic<\/p>\n<\/li>\n<li class=\"\" data-start=\"3437\" data-end=\"3527\">\n<p class=\"\" data-start=\"3439\" data-end=\"3527\">creation of CRISPR chips - devices integrated into gadgets that analyze biomaterials<\/p>\n<\/li>\n<li class=\"\" data-start=\"3528\" data-end=\"3571\">\n<p class=\"\" data-start=\"3530\" data-end=\"3571\">reducing the cost of tests to $1\u20132<\/p>\n<\/li>\n<\/ul>\n<blockquote data-start=\"3573\" data-end=\"3753\">\n<p class=\"\" data-start=\"3575\" data-end=\"3753\">\u00abIn the next 5 years, CRISPR diagnostics will become part of the annual checkup. We will be able to detect diseases before symptoms appear,\u00bb predicts Dr. Leila Morales, a geneticist at UCSF.<\/p>\n<\/blockquote>\n<h3 class=\"\" data-start=\"3755\" data-end=\"3779\"><strong>Limitations and challenges<\/strong><\/h3>\n<ul data-start=\"3781\" data-end=\"4000\">\n<li class=\"\" data-start=\"3781\" data-end=\"3821\">\n<p class=\"\" data-start=\"3783\" data-end=\"3821\">difficulty of certifying new tests<\/p>\n<\/li>\n<li class=\"\" data-start=\"3822\" data-end=\"3886\">\n<p class=\"\" data-start=\"3824\" data-end=\"3886\">the need for fine-tuning of each diagnostic set<\/p>\n<\/li>\n<li class=\"\" data-start=\"3887\" data-end=\"3949\">\n<p class=\"\" data-start=\"3889\" data-end=\"3949\">sensitivity to storage and transportation conditions of reagents<\/p>\n<\/li>\n<li class=\"\" data-start=\"3950\" data-end=\"4000\">\n<p class=\"\" data-start=\"3952\" data-end=\"4000\">risk of errors in unqualified use<\/p>\n<\/li>\n<\/ul>\n<p>CRISPR diagnostics is a combination of precision, speed, and innovation. Its emergence makes laboratory tests more accessible, adaptable, and personalized. This is not just a technology \u2014 it is a paradigm shift in global medicine.<\/p>","protected":false},"excerpt":{"rendered":"<p>Until recently, CRISPR was associated exclusively with genome editing. However, today this tool is used not only to change DNA, but<\/p>","protected":false},"author":627,"featured_media":8756,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-8755","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>CRISPR-\u0434\u0456\u0430\u0433\u043d\u043e\u0441\u0442\u0438\u043a\u0430 \u2014 \u043d\u043e\u0432\u0430 \u0435\u0440\u0430 \u0432\u0438\u044f\u0432\u043b\u0435\u043d\u043d\u044f \u0445\u0432\u043e\u0440\u043e\u0431<\/title>\n<meta name=\"description\" content=\"\u0429\u043e \u0442\u0430\u043a\u0435 CRISPR-\u0434\u0456\u0430\u0433\u043d\u043e\u0441\u0442\u0438\u043a\u0430? \u042f\u043a \u0432\u043e\u043d\u0430 \u043f\u0440\u0430\u0446\u044e\u0454, \u0443 \u0447\u043e\u043c\u0443 \u0457\u0457 \u043f\u0435\u0440\u0435\u0432\u0430\u0433\u0438, \u0434\u0435 \u0432\u0436\u0435 \u0437\u0430\u0441\u0442\u043e\u0441\u043e\u0432\u0443\u0454\u0442\u044c\u0441\u044f, \u0456 \u0447\u043e\u043c\u0443 \u0432\u043e\u043d\u0430 \u0437\u043c\u0456\u043d\u044e\u0454 \u043f\u0440\u0430\u0432\u0438\u043b\u0430 \u0433\u0440\u0438 \u0443 \u043c\u0435\u0434\u0438\u0447\u043d\u0456\u0439 \u0434\u0456\u0430\u0433\u043d\u043e\u0441\u0442\u0438\u0446\u0456\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medilab.km.ua\/en\/crispr-diagnostics-revolutionizes-the-accuracy-and-speed-of-disease-detection\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"CRISPR-\u0434\u0456\u0430\u0433\u043d\u043e\u0441\u0442\u0438\u043a\u0430 \u2014 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