{"id":9076,"date":"2024-10-23T10:00:18","date_gmt":"2024-10-23T07:00:18","guid":{"rendered":"https:\/\/medilab.km.ua\/?p=9076"},"modified":"2026-04-20T18:55:22","modified_gmt":"2026-04-20T15:55:22","slug":"multiplex-analysis-accurate-diagnostics-at-once-for-several-indicators","status":"publish","type":"post","link":"https:\/\/medilab.km.ua\/en\/multiplex-analysis-accurate-diagnostics-at-once-for-several-indicators\/","title":{"rendered":"Multiplex analysis: accurate diagnosis based on several indicators at once"},"content":{"rendered":"<h2 class=\"\" data-start=\"79\" data-end=\"115\"><strong data-start=\"82\" data-end=\"115\">What is multiplex analysis?<\/strong><\/h2>\n<p class=\"\" data-start=\"348\" data-end=\"620\">Multiplex analysis is a modern high-tech technique that allows for the simultaneous examination of dozens, and sometimes hundreds, of biomarkers within a single biological sample. Due to this ability, the method has found wide application in medical diagnostics, science, and pharmacology.<\/p>\n<p class=\"\" data-start=\"622\" data-end=\"946\">The technique is based on microarray, biochip, multiplex PCR (qPCR) or fluorescently labeled microsphere technologies. Each area of the chip or bead is responsible for a specific marker \u2014 protein, DNA, RNA or other analyte. When a reagent is added to the sample, the system analyzes many parameters at once, reading the result automatically.<\/p>\n<h2 class=\"\" data-start=\"637\" data-end=\"683\"><strong data-start=\"640\" data-end=\"683\">What are the advantages of multiplex analysis?<\/strong><\/h2>\n<ul data-start=\"685\" data-end=\"1068\">\n<li class=\"\" data-start=\"685\" data-end=\"767\">\n<p class=\"\" data-start=\"1013\" data-end=\"1117\">The advantages of multiplex analysis are not only in convenience. It is about a qualitatively different level of medical accuracy:<\/p>\n<ul data-start=\"1119\" data-end=\"1550\">\n<li class=\"\" data-start=\"1119\" data-end=\"1221\">\n<p class=\"\" data-start=\"1121\" data-end=\"1221\"><strong data-start=\"1121\" data-end=\"1139\">Complexity:<\/strong> you get several indicators at once without additional tests or repeat visits<\/p>\n<\/li>\n<li class=\"\" data-start=\"1222\" data-end=\"1309\">\n<p class=\"\" data-start=\"1224\" data-end=\"1309\"><strong data-start=\"1224\" data-end=\"1242\">Time saving:<\/strong> instead of dozens of separate tests, one with results in 1\u20132 days<\/p>\n<\/li>\n<li class=\"\" data-start=\"1310\" data-end=\"1383\">\n<p class=\"\" data-start=\"1312\" data-end=\"1383\"><strong data-start=\"1312\" data-end=\"1335\">Less biomaterial:<\/strong> one tube of blood or even a smear is enough<\/p>\n<\/li>\n<li class=\"\" data-start=\"1384\" data-end=\"1483\">\n<p class=\"\" data-start=\"1386\" data-end=\"1483\"><strong data-start=\"1386\" data-end=\"1406\">Higher sensitivity:<\/strong> some multiplex methods detect biomarkers at ultra-low concentrations<\/p>\n<\/li>\n<li class=\"\" data-start=\"1484\" data-end=\"1550\">\n<p class=\"\" data-start=\"1486\" data-end=\"1550\"><strong data-start=\"1486\" data-end=\"1504\">Automation:<\/strong> minimizes human error and measurement errors<\/p>\n<\/li>\n<\/ul>\n<p class=\"\" data-start=\"1552\" data-end=\"1725\">This is especially important in cases where symptoms are nonspecific or the patient is difficult to undergo advanced diagnostics by traditional methods (e.g., children, the elderly, or the seriously ill).<\/p>\n<p class=\"\" data-start=\"687\" data-end=\"767\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-9078\" src=\"https:\/\/medilab.km.ua\/wp-content\/themes\/woodmart\/images\/lazy.svg\" data-src=\"https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/04\/11-15.jpg\" alt=\"doctor explaining multiplex panel results to patient\" width=\"1200\" height=\"801\" srcset=\"\" data-srcset=\"https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/04\/11-15.jpg 1200w, https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/04\/11-15-150x100.jpg 150w, https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/04\/11-15-600x401.jpg 600w, https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/04\/11-15-300x200.jpg 300w, https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/04\/11-15-1024x684.jpg 1024w, https:\/\/medilab.km.ua\/wp-content\/uploads\/2025\/04\/11-15-768x513.jpg 768w\" sizes=\"auto, (max-width: 1200px) 100vw, 1200px\" \/><\/p>\n<\/li>\n<\/ul>\n<h2 class=\"\" data-start=\"1070\" data-end=\"1127\"><strong data-start=\"1073\" data-end=\"1127\">In which cases is multiplex analysis used?<\/strong><\/h2>\n<ul data-start=\"1129\" data-end=\"1624\">\n<li class=\"\" data-start=\"1129\" data-end=\"1243\">\n<p class=\"\" data-start=\"1131\" data-end=\"1243\"><strong data-start=\"1131\" data-end=\"1159\">Infectious diseases:<\/strong> detection of several pathogens simultaneously (for example, <a href=\"https:\/\/medilab.km.ua\/en\/what-is-torch-infection-and-when-should-i-take-the-test-and-why-is-it-important\/\">TORCH complex<\/a>, PCR for STIs)<\/p>\n<\/li>\n<li class=\"\" data-start=\"1244\" data-end=\"1337\">\n<p class=\"\" data-start=\"1246\" data-end=\"1337\"><strong data-start=\"1246\" data-end=\"1262\">Allergology:<\/strong> allergy panels with dozens of allergens (food, household, pollen, etc.)<\/p>\n<\/li>\n<li class=\"\" data-start=\"1338\" data-end=\"1440\">\n<p class=\"\" data-start=\"1340\" data-end=\"1440\"><strong data-start=\"1340\" data-end=\"1354\">Oncology:<\/strong> tumor marker panels that allow for the simultaneous assessment of several types of tumor risk<\/p>\n<\/li>\n<li class=\"\" data-start=\"1441\" data-end=\"1534\">\n<p class=\"\" data-start=\"1443\" data-end=\"1534\"><strong data-start=\"1443\" data-end=\"1456\"><a href=\"https:\/\/medilab.km.ua\/en\/price\/?3325_product_cat%5B0%5D=219&amp;3325_device=laptop&amp;3325_filtered=true\">Genetics<\/a>:<\/strong> mutation screening, genetic predisposition analysis, pharmacogenetic testing<\/p>\n<\/li>\n<li class=\"\" data-start=\"1535\" data-end=\"1624\">\n<p class=\"\" data-start=\"1537\" data-end=\"1624\"><strong data-start=\"1537\" data-end=\"1565\">Autoimmune diseases:<\/strong> autoantibody panels for comprehensive assessment of immune status<\/p>\n<\/li>\n<\/ul>\n<h2 class=\"\" data-start=\"2476\" data-end=\"2518\"><strong data-start=\"2479\" data-end=\"2518\">Example: ALEX2 allergy panel<\/strong><\/h2>\n<p class=\"\" data-start=\"2520\" data-end=\"2758\">One of the most well-known platforms, ALEX2, allows you to test your body&#039;s response to 300+ allergens and allergenic components. This test allows you to distinguish true allergies from cross-allergies, determine IgE levels, and detail the risk of anaphylaxis.<\/p>\n<p class=\"\" data-start=\"2760\" data-end=\"2895\">Multiplex allergy tests are especially useful for children, people with atopic dermatitis, bronchial asthma, and polyvalent allergies.<\/p>\n<h2 class=\"\" data-start=\"1984\" data-end=\"2025\"><strong data-start=\"1987\" data-end=\"2025\">How multiplex technology works<\/strong><\/h2>\n<p class=\"\" data-start=\"2027\" data-end=\"2353\">Most multiplex platforms use the principle of microchips, microspheres, or fluorescent labeling. For example, dozens of zones can be fixed on a single carrier, each of which is responsible for a separate marker. The sample is applied to the chip, the reaction is started, and the system automatically reads the results for all indicators at once.<\/p>\n<p class=\"\" data-start=\"2355\" data-end=\"2402\">This principle is used by technologies such as:<\/p>\n<ul data-start=\"2404\" data-end=\"2512\">\n<li class=\"\" data-start=\"2404\" data-end=\"2415\">\n<p class=\"\" data-start=\"2406\" data-end=\"2415\">Luminex<\/p>\n<\/li>\n<li class=\"\" data-start=\"2416\" data-end=\"2435\">\n<p class=\"\" data-start=\"2418\" data-end=\"2435\">ELISA multiplex<\/p>\n<\/li>\n<li class=\"\" data-start=\"2436\" data-end=\"2454\">\n<p class=\"\" data-start=\"2438\" data-end=\"2454\">DNA microarray<\/p>\n<\/li>\n<li class=\"\" data-start=\"2455\" data-end=\"2512\">\n<p class=\"\" data-start=\"2457\" data-end=\"2512\">qPCR Multiplex (real-time PCR for multiple targets)<\/p>\n<\/li>\n<\/ul>\n<h2 class=\"\" data-start=\"2514\" data-end=\"2588\"><strong data-start=\"2517\" data-end=\"2588\">Table: Comparison of multiplex analysis with traditional methods<\/strong><\/h2>\n<div class=\"pointer-events-none relative left-[50%] flex w-[100cqw] translate-x-[-50%] justify-center *:pointer-events-auto\">\n<div class=\"tableContainer horzScrollShadows\">\n<table class=\"min-w-full\" data-start=\"2590\" data-end=\"3324\">\n<thead data-start=\"2590\" data-end=\"2694\">\n<tr data-start=\"2590\" data-end=\"2694\">\n<th data-start=\"2590\" data-end=\"2622\"><strong data-start=\"2592\" data-end=\"2604\">Criterion<\/strong><\/th>\n<th data-start=\"2622\" data-end=\"2659\"><strong data-start=\"2624\" data-end=\"2649\">Multiplex analysis<\/strong><\/th>\n<th data-start=\"2659\" data-end=\"2694\"><strong data-start=\"2661\" data-end=\"2682\">Traditional method<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody data-start=\"2800\" data-end=\"3324\">\n<tr data-start=\"2800\" data-end=\"2903\">\n<td class=\"\" data-start=\"2800\" data-end=\"2831\">Number of indicators<\/td>\n<td class=\"\" data-start=\"2831\" data-end=\"2868\">10\u2013100+ simultaneously<\/td>\n<td class=\"\" data-start=\"2868\" data-end=\"2903\">1 for analysis<\/td>\n<\/tr>\n<tr data-start=\"2904\" data-end=\"3010\">\n<td class=\"\" data-start=\"2904\" data-end=\"2937\">Speed of getting results<\/td>\n<td class=\"\" data-start=\"2937\" data-end=\"2974\">1\u20132 days<\/td>\n<td class=\"\" data-start=\"2974\" data-end=\"3010\">3\u20137 days (in case of multiple tests)<\/td>\n<\/tr>\n<tr data-start=\"3011\" data-end=\"3114\">\n<td class=\"\" data-start=\"3011\" data-end=\"3042\">Biomaterial costs<\/td>\n<td class=\"\" data-start=\"3042\" data-end=\"3079\">minimal<\/td>\n<td class=\"\" data-start=\"3079\" data-end=\"3114\">for each test separately<\/td>\n<\/tr>\n<tr data-start=\"3115\" data-end=\"3218\">\n<td class=\"\" data-start=\"3115\" data-end=\"3146\">Application in diagnostics<\/td>\n<td class=\"\" data-start=\"3146\" data-end=\"3183\">complex states<\/td>\n<td class=\"\" data-start=\"3183\" data-end=\"3218\">narrow-profile indicators<\/td>\n<\/tr>\n<tr data-start=\"3219\" data-end=\"3324\">\n<td class=\"\" data-start=\"3219\" data-end=\"3250\">Cost<\/td>\n<td class=\"\" data-start=\"3250\" data-end=\"3287\">profitable on several indicators<\/td>\n<td class=\"\" data-start=\"3287\" data-end=\"3324\">more expensive in total for individual tests<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Multiplex analysis is an example of how technology is changing medicine: faster, more accurate, and less expensive. Whether you want to get a complete picture of your health or are looking for the causes of complex symptoms, this method can be the key to understanding.<\/p>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>What is multiplex analysis? Multiplex analysis is a modern high-tech technique that allows you to simultaneously examine dozens, and sometimes hundreds<\/p>","protected":false},"author":627,"featured_media":9077,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-9076","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog"],"_links":{"self":[{"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/posts\/9076","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/users\/627"}],"replies":[{"embeddable":true,"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/comments?post=9076"}],"version-history":[{"count":0,"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/posts\/9076\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/media\/9077"}],"wp:attachment":[{"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/media?parent=9076"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/categories?post=9076"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/tags?post=9076"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}