{"id":9990,"date":"2025-07-08T01:08:25","date_gmt":"2025-07-07T22:08:25","guid":{"rendered":"https:\/\/medilab.km.ua\/?p=9990"},"modified":"2026-04-20T18:53:39","modified_gmt":"2026-04-20T15:53:39","slug":"laser-spectroscopy-as-light-helps-us-to-see-the-invisible","status":"publish","type":"post","link":"https:\/\/medilab.km.ua\/en\/laser-spectroscopy-as-light-helps-us-to-see-the-invisible\/","title":{"rendered":"Laser spectroscopy: how light helps us see the invisible"},"content":{"rendered":"
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Imagine looking at a drop of water. It appears clear, but inside it may be metal impurities, pesticide residues, or even traces of bacteria. You can't see this with the naked eye. This is where the laser spectroscopy<\/strong> \u2014 a method that converts light on the deep diagnostic tool<\/strong>, capable of recognizing the composition of any substance with incredible accuracy. From NASA laboratories to doctors' offices, this technology is changing the way we think about analysis.<\/p>\n

What is laser spectroscopy?<\/h2>\n

Laser spectroscopy is a highly sensitive method of analyzing substances based on interaction of laser radiation with atoms or molecules of the sample<\/strong>. As a result of this interaction, absorption, scattering, or luminescence spectrum<\/strong>, which can determine the chemical composition, concentration, and structural features of a substance.<\/p>\n

Basic principles of the method<\/h2>\n

The method is based on the fact that an atom or molecule absorbs or emits light at strictly defined wavelengths<\/strong>. These waves form a spectrum, like a "fingerprint" of a substance\u2014unique to each element or compound.<\/p>\n

A typical process looks like this:<\/p>\n

    \n
  1. \n

    The laser beam is focused on the sample.<\/p>\n<\/li>\n

  2. \n

    Radiation interacts with matter (causing ionization, fluorescence, or scattering).<\/p>\n<\/li>\n

  3. \n

    Detectors record a spectrum \u2014 a set of wavelengths.<\/p>\n<\/li>\n

  4. \n

    The program analyzes the spectrum by comparing it with reference databases.<\/p>\n<\/li>\n<\/ol>\n

    Main types of laser spectroscopy<\/h2>\n
    \n
    \n\n\n\n\n\n\n\n\n
    Method name<\/th>\nFull name<\/th>\nWorking principle<\/th>\nMain applications<\/th>\n<\/tr>\n<\/thead>\n
    LIBS<\/strong><\/td>\nLaser-Induced Breakdown Spectroscopy<\/td>\nThe laser creates plasma on the sample surface, the plasma spectrum is analyzed<\/td>\nGeology, metallurgy, pharmacology<\/td>\n<\/tr>\n
    LIFS<\/strong><\/td>\nLaser-Induced Fluorescence Spectroscopy<\/td>\nLaser-induced fluorescence recording<\/td>\nOncodiagnostics, toxicology<\/td>\n<\/tr>\n
    TDLAS<\/strong><\/td>\nTunable Diode Laser Absorption Spectroscopy<\/td>\nMeasuring the absorption of laser light of a specific wavelength<\/td>\nGas analysis, emission monitoring<\/td>\n<\/tr>\n
    CARS<\/strong><\/td>\nCoherent Anti-Stokes Raman Spectroscopy<\/td>\nNonlinear spectroscopy based on the Raman effect<\/td>\nBiomolecular research, biophysics<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
    <\/div>\n<\/div>\n<\/div>\n<\/div>\n

    Why is laser spectroscopy a breakthrough?<\/h2>\n
      \n
    • \n

      Contactless<\/strong>: no need to take a sample or destroy the specimen.<\/p>\n<\/li>\n

    • \n

      Efficiency<\/strong>: Results can be obtained within a few seconds.<\/p>\n<\/li>\n

    • \n

      Versatility<\/strong>: suitable for the analysis of solid, liquid and gaseous media.<\/p>\n<\/li>\n

    • \n

      High sensitivity<\/strong>: capable of detecting substances in trace concentrations.<\/p>\n<\/li>\n<\/ul>\n

      This makes the method indispensable in situations where speed and accuracy are critical<\/strong>, for example, in the analysis of toxic substances or the detection of biomarkers of diseases.<\/p>\n

      \"spectrum<\/p>\n

      Where is it used?<\/h2>\n

      1. Medicine<\/h3>\n

      Laser biospectroscopy uses fluorescence to detect cancer cells<\/strong>, pathogenic proteins<\/strong> or signs of inflammation<\/strong> in biological fluids. This allows for preliminary diagnostic results without biopsy<\/strong>.<\/p>\n

      2. Ecology<\/h3>\n

      TDLAS-based systems are used to detect polluting gases in the air<\/strong> \u2014 methane, ammonia, nitrogen oxides \u2014 with an accuracy of parts per million.<\/p>\n

      3. Industry<\/h3>\n

      In metallurgy and pharmaceuticals, laser spectroscopy is used for quality control of raw materials and finished products<\/strong> \u2014 without the need to take samples manually.<\/p>\n

      4. Space<\/h3>\n

      NASA is actively using LIBS in Mars rovers (e.g., Curiosity<\/strong>) to examine the soil and detect traces of life or water.<\/p>\n

      Example: how laser spectroscopy helps detect cancer<\/h2>\n

      During fluorescence diagnostics, a laser causes the tissue to glow. Healthy and tumor tissue have different spectral profiles<\/strong>. This allows:<\/p>\n

        \n
      • \n

        to notice oncomutation at an early stage;<\/p>\n<\/li>\n

      • \n

        monitor response to treatment;<\/p>\n<\/li>\n

      • \n

        reduce the number of invasive procedures.<\/p>\n<\/li>\n<\/ul>\n

        Such methods become the basis non-invasive medicine of the future<\/strong>.<\/p>\n

        Strengths and limitations<\/h2>\n

        Advantages:<\/strong><\/p>\n

          \n
        • \n

          Extreme analysis speed.<\/p>\n<\/li>\n

        • \n

          Possibility in vivo research<\/strong>.<\/p>\n<\/li>\n

        • \n

          High selectivity and sensitivity.<\/p>\n<\/li>\n<\/ul>\n

          Disadvantages:<\/strong><\/p>\n

            \n
          • \n

            High cost of equipment.<\/p>\n<\/li>\n

          • \n

            The need for calibration.<\/p>\n<\/li>\n

          • \n

            Interference is possible when analyzing complex mixtures.<\/p>\n<\/li>\n<\/ul>\n

            Technology prospects<\/h2>\n

            Every year, laser spectroscopy becomes more accessible: the size of the equipment is decreasing, the software is improving, and artificial intelligence is being integrated. In the next 5\u201310 years, mass use of portable spectroscopes is expected in:<\/p>\n

              \n
            • \n

              clinical practice;<\/p>\n<\/li>\n

            • \n

              food control;<\/p>\n<\/li>\n

            • \n

              personal environmental safety.<\/p>\n<\/li>\n<\/ul>\n

              Laser spectroscopy is not just a scientific method, but a real tool of the future<\/strong>, which is already operating in a wide variety of areas today \u2014 from laboratories to medical centers, from factories to orbital stations. This is an example of how science helps to see what remained hidden to the eye and make the world safer and more understandable.<\/p>","protected":false},"excerpt":{"rendered":"

              Imagine looking at a drop of water. It appears clear, but inside it may be metal impurities, pesticide residues, or<\/p>","protected":false},"author":627,"featured_media":9991,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-9990","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"yoast_head":"\n\u041b\u0430\u0437\u0435\u0440\u043d\u0430 \u0441\u043f\u0435\u043a\u0442\u0440\u043e\u0441\u043a\u043e\u043f\u0456\u044f: \u043f\u0440\u0438\u043d\u0446\u0438\u043f \u0434\u0456\u0457, \u0432\u0438\u0434\u0438, \u043f\u0440\u0438\u043a\u043b\u0430\u0434\u0438 \u0437\u0430\u0441\u0442\u043e\u0441\u0443\u0432\u0430\u043d\u043d\u044f<\/title>\n<meta name=\"description\" content=\"\u0429\u043e \u0442\u0430\u043a\u0435 \u043b\u0430\u0437\u0435\u0440\u043d\u0430 \u0441\u043f\u0435\u043a\u0442\u0440\u043e\u0441\u043a\u043e\u043f\u0456\u044f? \u042f\u043a 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