{"id":3535,"date":"2021-03-12T08:59:33","date_gmt":"2021-03-12T06:59:33","guid":{"rendered":"https:\/\/medilab.km.ua\/?post_type=product&#038;p=3535"},"modified":"2026-11-02T23:29:03","modified_gmt":"2026-11-02T21:29:03","slug":"detection-of-lactose-deficiency-genetics-of-lactose-metabolism-real-time-pcr","status":"publish","type":"product","link":"https:\/\/medilab.km.ua\/en\/analizy\/genetychni-doslidzhennya\/vyznachennya-laktaznoyi-nedostatnosti-genetyka-metabolizmu-laktozy-real-time-pcr\/","title":{"rendered":"Genetics. PCR. Lactose Metabolism (Determination of lactase deficiency)"},"content":{"rendered":"<ul>\n<li>\n<p data-path-to-node=\"1\"><b data-path-to-node=\"1\" data-index-in-node=\"0\">Genetic study of lactose metabolism (PCR)<\/b> \u2014 is a highly accurate molecular biological analysis that allows you to detect a hereditary predisposition to primary lactase deficiency. This is a condition in which the body&#039;s production of the lactase enzyme, which is necessary for the breakdown of milk sugar (lactose), decreases with age. The study is carried out by analyzing the regulatory region <b data-path-to-node=\"1\" data-index-in-node=\"372\">MCM6 gene<\/b> (which controls the LCT gene, which is responsible for lactase synthesis).<\/p>\n<p data-path-to-node=\"2\">Since this is a genetic analysis, it <b data-path-to-node=\"2\" data-index-in-node=\"36\">the result does not change throughout life<\/b>, so it is enough to take the study only once.<\/p>\n<h4 data-path-to-node=\"3\">Investigated indicators and interpretation of results (Genotypes)<\/h4>\n<p data-path-to-node=\"4\">The result of the analysis is the detection of one of three possible genetic polymorphisms (genotypes) in the C(-13910)T marker of the MCM6 gene:<\/p>\n<ol start=\"1\" data-path-to-node=\"5\">\n<li>\n<p data-path-to-node=\"5,0,0\"><b data-path-to-node=\"5,0,0\" data-index-in-node=\"0\">Genotype C\/C (homozygous for the wild-type allele)<\/b><\/p>\n<ul data-path-to-node=\"5,0,1\">\n<li>\n<p data-path-to-node=\"5,0,1,0,0\"><b data-path-to-node=\"5,0,1,0,0\" data-index-in-node=\"0\">What does it mean:<\/b> Primary lactase deficiency (adult hypolactasia) is genetically determined. With age, the activity of the lactase enzyme significantly decreases or stops.<\/p>\n<\/li>\n<li>\n<p data-path-to-node=\"5,0,1,1,0\"><b data-path-to-node=\"5,0,1,1,0\" data-index-in-node=\"0\">Clinical manifestations:<\/b> High risk of dairy intolerance. Consumption of unfermented milk leads to severe discomfort (bloating, pain, diarrhea). The patient is advised to follow a lactose-free diet.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li>\n<p data-path-to-node=\"5,1,0\"><b data-path-to-node=\"5,1,0\" data-index-in-node=\"0\">Genotype C\/T (heterozygote)<\/b><\/p>\n<ul data-path-to-node=\"5,1,1\">\n<li>\n<p data-path-to-node=\"5,1,1,0,0\"><b data-path-to-node=\"5,1,1,0,0\" data-index-in-node=\"0\">What does it mean:<\/b> A variant of the norm. The lactase enzyme is produced in sufficient quantities to digest moderate portions of lactose.<\/p>\n<\/li>\n<li>\n<p data-path-to-node=\"5,1,1,1,0\"><b data-path-to-node=\"5,1,1,1,0\" data-index-in-node=\"0\">Clinical manifestations:<\/b> Milk is well tolerated, however, against the background of stress, intestinal infections, or inflammatory diseases of the gastrointestinal tract, temporary (secondary) lactase deficiency may develop.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li>\n<p data-path-to-node=\"5,2,0\"><b data-path-to-node=\"5,2,0\" data-index-in-node=\"0\">Genotype T\/T (homozygote for the mutant allele)<\/b><\/p>\n<ul data-path-to-node=\"5,2,1\">\n<li>\n<p data-path-to-node=\"5,2,1,0,0\"><b data-path-to-node=\"5,2,1,0,0\" data-index-in-node=\"0\">What does it mean:<\/b> Absolutely normal. The activity of the lactase enzyme remains at a high level throughout life.<\/p>\n<\/li>\n<li>\n<p data-path-to-node=\"5,2,1,1,0\"><b data-path-to-node=\"5,2,1,1,0\" data-index-in-node=\"0\">Clinical manifestations:<\/b> Excellent tolerance of dairy products. If a patient has digestive problems after consuming milk, their cause should be sought elsewhere (for example, allergy to cow&#039;s milk protein, dysbacteriosis, etc.).<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n<h4 data-path-to-node=\"6\">Indications for appointment<\/h4>\n<ul data-path-to-node=\"7\">\n<li>\n<p data-path-to-node=\"7,0,0\">Discomfort in the gastrointestinal tract after consuming milk and dairy products (flatulence, cramps, bloating, diarrhea, nausea).<\/p>\n<\/li>\n<li>\n<p data-path-to-node=\"7,1,0\">Differential diagnosis of lactase deficiency and other gastrointestinal diseases (irritable bowel syndrome, Crohn&#039;s disease, celiac disease).<\/p>\n<\/li>\n<li>\n<p data-path-to-node=\"7,2,0\">Assessing the risk of developing osteoporosis (since lactose intolerance often leads to calcium deficiency due to avoidance of dairy products).<\/p>\n<\/li>\n<li>\n<p data-path-to-node=\"7,3,0\">Drawing up an individual nutrition plan.<\/p>\n<\/li>\n<li>\n<p data-path-to-node=\"7,4,0\">Comprehensive examination for chronic dermatitis or unexplained allergic reactions.<\/p>\n<\/li>\n<\/ul>\n<h4 data-path-to-node=\"8\">Preparation for the study<\/h4>\n<ul data-path-to-node=\"9\">\n<li>\n<p data-path-to-node=\"9,0,0\"><b data-path-to-node=\"9,0,0\" data-index-in-node=\"0\">If the biomaterial is venous blood:<\/b> It is taken strictly on an empty stomach (last meal 8-12 hours before the analysis). You can drink plain water.<\/p>\n<\/li>\n<li>\n<p data-path-to-node=\"9,1,0\"><b data-path-to-node=\"9,1,0\" data-index-in-node=\"0\">If the biomaterial is a buccal scraping (swab from the inner surface of the cheek):<\/b> 2 hours before the sample is taken, you should not eat, drink, brush your teeth, smoke, or chew gum. Before the procedure, rinse your mouth with clean water.<\/p>\n<\/li>\n<\/ul>\n<h4 data-path-to-node=\"10\">Important notes<\/h4>\n<p data-path-to-node=\"11\">Genetic testing results are not a definitive diagnosis. A final diagnosis and dietary recommendations should be made by a gastroenterologist, internist, pediatrician, or dietitian based on a comprehensive assessment of clinical symptoms and medical history.<\/p>\n<\/li>\n<\/ul>","protected":false},"excerpt":{"rendered":"<p data-path-to-node=\"0\">Laboratory genetic testing to determine susceptibility to primary lactase deficiency (lactose intolerance) by analyzing polymorphisms of genes responsible for the synthesis of the lactase enzyme.<\/p>\n<ul data-path-to-node=\"1\">\n<li>\n<p data-path-to-node=\"1,0,0\"><b data-path-to-node=\"1,0,0\" data-index-in-node=\"0\">Biomaterial:<\/b> Venous blood (or buccal epithelium\/swab from the inner surface of the cheek)<\/p>\n<\/li>\n<li>\n<p data-path-to-node=\"1,1,0\"><b data-path-to-node=\"1,1,0\" data-index-in-node=\"0\">Research method:<\/b> Polymerase chain reaction (PCR)<\/p>\n<\/li>\n<li>\n<p data-path-to-node=\"1,2,0\"><b data-path-to-node=\"1,2,0\" data-index-in-node=\"0\">Appointment:<\/b> Diagnosis of genetically determined lactose intolerance, finding out the causes of bloating, abdominal pain and discomfort after consuming dairy products, selection of individual nutrition.<\/p>\n<\/li>\n<\/ul>","protected":false},"featured_media":15298,"comment_status":"open","ping_status":"closed","template":"","meta":{"_flow_reviewer_id":0,"_flow_review_status":""},"product_brand":[],"product_cat":[219,104],"product_tag":[],"class_list":["post-3535","product","type-product","status-publish","has-post-thumbnail","product_cat-genetychni-doslidzhennya","product_cat-diagnostyka-shlunkovo-kyshkovyh-zahvoryuvan","first","instock","shipping-taxable","purchasable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/product\/3535","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/comments?post=3535"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/media\/15298"}],"wp:attachment":[{"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/media?parent=3535"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/product_brand?post=3535"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/product_cat?post=3535"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/medilab.km.ua\/en\/wp-json\/wp\/v2\/product_tag?post=3535"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}