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Antioxidant level: what it is, how it is measured and why it is important to know it

Blood test for antioxidants in laboratory conditions
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Antioxidants — are molecules that protect the cells of our body from damage caused by free radicals. These radicals are formed as a result of normal metabolic processes or under the influence of harmful factors, such as ultraviolet light, pollution, smoking, stress, and poor diet. The level of antioxidants in the blood reflects the body's ability to resist oxidative stress, which is associated with the development of many chronic diseases — from cardiovascular to cancer.

What does an antioxidant level test show?

This test measures the total antioxidant capacity (TAC) in serum or plasma. It can be performed using different methods (e.g. FRAP, ORAC or TEAC), but the result is an estimate of the body's overall ability to neutralize free radicals. Levels of individual antioxidants, such as vitamins C, E, carotenoids, glutathione, selenium or enzymes such as superoxide dismutase, are often also assessed.

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Prevention of antioxidant deficiency

The main sources of antioxidants are:

  • Fruits and vegetables (berries, pomegranate, spinach, broccoli)

  • Products with vitamins A, C, E

  • Green tea, dark chocolate, turmeric

  • Omega-3 fatty acids

It is important to combine these products with a healthy lifestyle, normal sleep, and minimizing stress.

Foods rich in antioxidants

Methods for determining antioxidant levels

Both biochemical and instrumental methods are used for diagnosis. The most common tests are:

  • Total Antioxidant Capacity (TAC) Test — assesses the total ability of blood serum to neutralize free radicals.

  • Glutathione level determination — an endogenous antioxidant that plays a key role in cellular protection.

  • Analysis for antioxidant vitamins (A, C, E) — allows you to identify a deficiency that reduces overall antioxidant activity.

  • Assessment of the activity of the enzymes SOD (superoxide dismutase), catalase, and glutathione peroxidase.

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Factors that affect antioxidant levels

The main factors that reduce antioxidant levels include:

  • Chronic stress

  • Unbalanced diet

  • Long-term medication use

  • Smoking and alcohol

  • Exposure to toxic substances, heavy metals

  • Intense physical activity without recovery

People who often get sick, residents of cities with high levels of pollution, athletes, and pregnant women are at risk.

Who and when should undergo the study?

Examination recommended:

  • Patients with chronic diseases

  • People who take a lot of medications

  • For people with frequent colds or low immunity

  • For those planning wellness programs (detox, bioenergetic support)

  • Women during pregnancy or menopause

How to prepare for the analysis

  • Blood sampling is performed on an empty stomach, in the morning.

  • It is advisable to avoid intense physical activity and alcohol for 1–2 days.

  • It is not recommended to take antioxidant vitamin supplements without consulting a doctor.

  • In 12 hours — only water

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Results and their interpretation

The level of antioxidant activity is estimated in conventional units. High values may indicate excessive intake of vitamin supplements or a specific response of the body to the inflammatory process. Reduced levels signal increased oxidative stress and the need for dietary, lifestyle or drug support.

In modern medicine, antioxidant capacity analysis is not only a diagnostic tool, but also a way to prevent many diseases. It should be considered an important link in the system of personalized medicine, which allows assessing the body's reserves and adjusting risks at early stages.

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