Bacteriological examination of breast milk with identification of pathogens and determination of antibiogram using automatic analyzers AF300; Scan4000 according to EUCAST methodology (collection ONLY!!! in the laboratory)

Bacteriological examination of breast milk and determination of sensitivity to antibiotics is an important stage in ensuring the health and proper development of newborns. This procedure is carried out in a laboratory environment and allows you to detect the presence of pathogenic microorganisms in breast milk, as well as determine their sensitivity to antibiotics. Below we will consider the process of conducting a bacteriological examination and determining sensitivity to antibiotics on an automatic analyzer.

1. Collection of material: Breast milk culture is performed only in a laboratory environment under sterile conditions. The healthcare professional who collects the milk must have clean hands and use sterile instruments. Usually, a small amount of material is taken for testing.
2. Sample preparation: The breast milk sample is carefully labeled and prepared for analysis. It is usually diluted with special solutions to obtain an accurate result.
3. Bacterial screening: After preparing the sample material, it is plated on nutrient media that promote bacterial growth. This procedure is performed on special agars.
4. Bacterial cultivation: The sample is placed in an incubator where it is left for several days for the bacteria to grow.
5. Antibiotic susceptibility testing: The resulting bacterial colonies are tested for antibiotic susceptibility. This is an important step in determining the most effective treatment.
6. Obtaining Results: After the culture and antibiotic sensitivity testing are complete, the results are obtained. This allows you to determine whether the breast milk needs to be treated with antibiotics and choose the most effective drug.

It is important to emphasize that the bacteriological examination of breast milk is intended to ensure safe and healthy feeding of the newborn. The information obtained as a result of such an examination may be important for choosing the optimal treatment method and preventing possible complications.

The general information provided in this text provides a general overview of the process of bacteriological examination of human milk and determination of antibiotic susceptibility. The details of the procedure may vary depending on the specific laboratory protocol and equipment used for the analysis.