Bacteriological examination of the nail plate for fungal flora (genus Candida) with determination of the antimycoticogram using automatic analyzers AF300; Scan4000 according to the EUCAST, CLSI methodology

Nail plates can become a real “home” for various microorganisms, including fungi and bacteria. In order to determine whether fungal microflora is present on the nails and to determine its sensitivity to antibiotics, a bacteriological analysis of the biomaterial is performed on a specialized automatic analyzer. Let's look at this process in more detail.

1. Biomaterial collection: The first step is to collect the nail plate or nail fragments. Using a special instrument, the doctor takes a sample of the biomaterial and thoroughly disinfects the area to avoid contamination.
2. Sample preparation: The collected sample is placed on a plate of agar or other fungal culture medium. This plate is placed in an incubator to create optimal conditions for fungal growth.
3. Fungal culture: Within a few days, fungal colonies appear on the agar. Each colony represents a separate strain of fungus. Sometimes it is difficult to identify fungi visually, so microscopic examination is necessary.
4. Determination of antibiotic sensitivity: Fungal colonies are selected and tested for their sensitivity to various antibiotics. This process helps determine which medications may be effective in treating the fungal infection.
5. Automatic analysis: Specialized analyzers are used that automatically determine the sensitivity of fungi to antibiotics. This allows you to quickly and accurately determine the optimal course of treatment.
6. Results and treatment: After the analysis is completed, the doctor receives the results and develops an individual treatment plan for the patient, taking into account the sensitivity of the fungi to antibiotics.

This process of bacteriological analysis of the nail plate for fungal microflora and determination of antibiotic sensitivity is important for the diagnosis and treatment of fungal nail infections. It allows for effective and individual treatment for each patient, ensuring rapid restoration of the condition of the nail plate and overall health.