Bacteriological examination of urogenital discharge with identification of pathogens and determination of antibiogram using automatic analyzers AF300; Scan4000 according to EUCAST methodology

Bacteriological culture of the microflora of the urogenital tract and determination of antibiotic sensitivity are important components of the clinical diagnosis and treatment of various infectious diseases that affect the urinary system and human genital organs. This method allows you to identify pathogenic microorganisms that may be the cause of the disease and determine which antibiotics are most effective for treating a particular infection.

The procedure for bacteriological culture and determination of antibiotic sensitivity may look like this:

1. Biomaterial collection: The patient provides a sample of biomaterial, such as urine, secretions from the mucous membrane of the urinary tract or genitals. The sample is collected in a sterile container.
2. Sample preparation: The sample undergoes pre-treatment where it is diluted and centrifuged to separate the bacteria from other components of the biomaterial.
3. Bacterial culture: The sample is spread on a nutrient medium or Petri dish and incubated under optimal temperature and humidity conditions. This allows the bacteria to grow and multiply.
4. Bacteria identification: After incubation, laboratory workers determine what types of bacteria are present in the sample. This can be done using microscopy and other bacteriological methods.
5. Determination of antibiotic sensitivity: For this, a diffusion method is used, for example, disk diffusion or the Microdivision method. Antibiotic disks are placed on a special Petri dish, and it is seen which ones create a zone of inhibition around the bacteria. The larger the zone of inhibition, the more sensitive the bacteria are to a given antibiotic.
6. Evaluation of results and choice of treatment: Laboratory results help determine which antibiotics are most effective in treating the infection. The doctor prescribes an antibiotic, choosing the one to which the identified bacteria are sensitive.

Bacterial culture and antibiotic sensitivity testing may take several days, but it is a very important step in the correct and effective treatment of urogenital tract infections. Inadequate treatment can lead to the development of antibiotic resistance in bacteria and prolong the duration of the infectious process. Therefore, it is important to follow the doctor's recommendations and wait for the results of the bacteriological study before starting treatment.