Bacteriological examination of the throat (tonsils) with identification of pathogens and determination of the antibiogram using automatic analyzers AF300; Scan4000 according to the EUCAST methodology

Bacteriological examination of the throat (tonsils) and determination of sensitivity to antibiotics are important procedures in laboratory medicine. This allows you to establish the presence and type of pathogenic bacteria in the patient's throat, as well as determine which antibiotics they are sensitive to. Let's look at this process in more detail.

1. Biopsy: The first step is to collect the biopsies. This can be done by an otolaryngologist or other qualified healthcare professional. They usually use disposable cotton swabs to take small impressions from the surface of the tonsils. The samples are sent to a medical laboratory for further testing.
2. Culture: The collected samples are placed on a special culture medium that promotes bacterial growth. This may be agar or another medium that is favorable for the growth of specific microorganisms.
3. Bacterial identification: After the bacteria grow on the culture medium, they are identified using special tests and methods. Lab technicians can determine what types of bacteria are present in the samples and whether they are pathogens that can cause throat infections.
4. Antibiotic susceptibility testing: After identifying the bacteria, an antibiotic susceptibility test is performed. This is done by plating disks with different antibiotics on an agar plate. If the bacteria are sensitive to a particular antibiotic, they will not grow around the disk with that antibiotic. If the bacteria are resistant to the antibiotic, they will grow around the disk.
5. Analysis of results: After all the necessary tests are performed, laboratory technicians analyze the results. They determine which bacteria are present in the samples and which antibiotics may be effective in treating a particular patient.
6. Report Preparation: Based on the test results, the laboratory prepares a report that is sent to the doctor who ordered the test. The doctor uses this information to prescribe the optimal treatment for the patient.

It is important to note that correctly identifying the type of bacteria and their sensitivity to antibiotics is a key step in treating throat infections. This process helps to avoid unnecessary antibiotic use and increases the effectiveness of treatment.

It is recommended to submit the material before the start of antibacterial therapy or 14 days after the end of the course of treatment.

The sample is taken before eating or drinking, or 2 hours after.