Bacteriological examination of ejaculate with identification of pathogens and determination of antibiogram using automatic analyzers AF300; Scan4000 according to EUCAST methodology (collection in the laboratory)

Bacteriological analysis of semen is an important component of the diagnosis and treatment of various diseases of the reproductive and genitourinary systems. This analysis allows you to detect the presence of pathogenic microorganisms in semen, as well as determine their sensitivity to antibiotics. Let's look at the process of bacteriological analysis of semen and its importance.

Preparation for analysis:

Before submitting sperm for analysis, you must follow some rules:

1. Hygiene measures: Before collecting sperm, it is necessary to follow hygiene rules. It is recommended to wash the genitals and hands before collecting the material.
2. Avoiding ejaculation before the test: It is necessary to abstain from sexual intercourse and ejaculation for 2-7 days before semen collection. This allows for more accurate results.

Material collection:

Sperm collection is performed in a laboratory. The patient transfers the sperm into a special container. The laboratory technician processes the obtained material, incubating it under appropriate conditions for bacterial growth.

Bacteriological analysis of semen:

Next, a bacteriological analysis of sperm is performed in the laboratory:

1. Sperm insemination: The prohibited material is spread onto special agar plates containing nutrient media for bacterial growth. This allows the isolation and identification of pathogenic microorganisms.
2. Determination of antibiotic sensitivity: After bacteria are detected in the semen, an antibiogram is performed. This study determines which antibiotics are effective against specific strains of bacteria. This is important for choosing the right treatment.

Importance of the study:

Bacteriological analysis of semen has several important clinical applications:

1. Diagnosis of infections: Allows you to detect the presence of infectious diseases such as prostatitis, epididymitis, sexually transmitted diseases and others.
2. Choosing antibiotics: Determining the sensitivity of bacteria to antibiotics helps to prescribe appropriate and effective treatment.
3. Post-treatment monitoring: The study is also used to assess the effectiveness of treatment and detect recurrent infections.

In conclusion, bacteriological analysis of semen is an important method for the diagnosis and treatment of infectious diseases of the reproductive sphere. It helps to identify problems in time and provide appropriate treatment, which is key to preserving the patient's health and reproductive function.