Bacteriological examination of urogenital discharge for fungal flora (genus Candida) with determination of antimycoticogram using automatic analyzers AF300; Scan4000 according to EUCAST,CLSI methodology

Bacteriological analysis of urogenital biomaterial to determine the presence and type of fungi of the genus Candida is an important component of the diagnosis of gynecological and urological diseases. Fungi of the genus Candida can cause vaginitis, thrush, as well as urological diseases, such as fungal cystitis. An automatic analyzer is used to determine sensitivity to antibiotics, which allows you to choose the most effective drug for treatment.

The procedure for taking biomaterial and seeding it for fungal flora includes the following stages:

1. Patient preparation: The patient must follow personal hygiene rules before the analysis.
2. Collection of biomaterial: A doctor or nurse collects biomaterial (vaginal or urethral secretions, urine) using special devices. The collected material is carefully labeled for later identification.
3. Analyzer preparation: The apparatus for analyzing biomaterial for microflora is prepared taking into account the type of analysis that needs to be performed (identification of Candida fungi and determination of their sensitivity to antibiotics).
4. Seeding of biomaterial: The collected biomaterial is placed in a special Petri dish or other container and seeded on nutrient media that promote fungal growth.
5. Fungal culture: Petri dishes or other vessels with biomaterial are placed in an incubator under optimal temperature and humidity conditions for fungal growth.
6. Fungal identification: After the fungi grow on nutrient media, they are identified using microscopy and biochemical tests. The species of fungi of the genus Candida is determined.
7. Determination of sensitivity to antibiotics: To determine the sensitivity of fungi to antibiotics, a special test is performed, which allows you to determine which antibiotics most effectively fight the identified fungi.
8. Analysis of results: The obtained data from the analysis of fungal flora and antibiotic sensitivity are used by the doctor to prescribe optimal treatment for the patient.

It is important to emphasize that proper diagnosis and treatment of fungal diseases are key to ensuring the health of patients. Accurate identification of fungi and determination of their sensitivity to antibiotics help in choosing the optimal treatment regimen and preventing disease recurrence.