Bacteriological examination of the nail pocket with identification of pathogens and determination of the antibiogram using automatic analyzers AF300; Scan4000 according to the EUCAST methodology

Culture of bacteriological contents of the nail pocket and determination of sensitivity to antibiotics: modern methods of studying biomaterial for microflora

Introduction

The study of the bacteriological content of the nail pocket and the determination of the sensitivity of microorganisms to antibiotics are important aspects of clinical diagnostics and treatment of infectious diseases. This process is simplified by the use of modern automatic analyzers, which allow you to quickly and accurately determine the microflora and its reaction to antibiotics. In this article, we will consider the procedure for conducting a bacteriological study of the nail pocket and determining the sensitivity to antibiotics on an automatic analyzer.

Preparation for the study

The first step in the study is proper preparation of the biomaterial. The nail pocket should be thoroughly cleaned and trimmed, while it is important to avoid contact with the nail or skin to avoid contamination with external microorganisms.

Obtaining biomaterial

Special sterile extractors are used to collect the biomaterial. Typically, the sample is taken by inserting the extractor into the nail pocket and gently rotating it to collect the secretions. The sample is transported to the laboratory as soon as possible for further research.

Bacteriological analysis

In the laboratory, the biomaterial is subjected to bacteriological analysis. First, the sample is placed on special bacterial culture media that promote the growth of microorganisms. Typically, agar loops with antimicrobial additives are used to help isolate specific types of bacteria.

Identification of microflora

After culturing the bacteria, identification of the species of microorganisms is carried out. Modern automated analyzers use various methods, such as mass spectrometry or polymerase chain reaction (PCR), to accurately identify the bacteria.

Determination of antibiotic sensitivity

After identifying microorganisms, their sensitivity to antibiotics is determined. This stage is performed by determining the minimum inhibitory concentration (MIC) of the antibiotics being tested. An automated analyzer allows for the accurate determination of the MIC for each antibiotic, which helps doctors choose the most effective drug for treatment.

Conclusion

The study of the bacteriological content of the nail pocket and the determination of sensitivity to antibiotics is an important stage in the diagnosis and treatment of infectious diseases. The use of modern automatic analyzers allows for a quick and accurate determination of microflora and its reaction to antibiotics, which makes this process more effective and safe for patients. It is important to emphasize that the correct preparation of the biomaterial and the observance of sterile conditions guarantee reliable research results.