Bacteriological examination of nasal passages with identification of pathogens and determination of antibiogram using automatic analyzers AF300; Scan4000 according to EUCAST methodology

Bacteriological examination of biomaterial from the nasal passages and determination of sensitivity to antibiotics is an important component of clinical diagnostics and treatment of various diseases. This method allows to determine the presence of pathogenic bacteria in the patient's nasal passages, as well as to establish which antibiotics may be effective for their treatment. In this article, we will consider the process of conducting bacteriological analysis and determining sensitivity to antibiotics on an automatic analyzer.

1. Collection of biomaterial: The first stage of the study is the collection of biomaterial from the patient's nasal passages. This can be done using a special type of cotton swab. The collected material should be processed with minimal intervention to preserve the integrity of the bacteria.
2. Bacterial culture: The obtained biomaterial is placed on a nutrient medium that promotes bacterial growth. This can be an agar plate or other suitable medium. After placement on the medium, the samples are incubated under appropriate temperature conditions for a certain time (usually 24-48 hours).
3. Determination of microflora: After incubation, the diversity and number of bacteria growing on the medium can be assessed. This step allows us to determine which species of bacteria are present in the biomaterial and their number.
4. Antibiotic susceptibility test: To determine the susceptibility of bacteria to antibiotics, antibiotic discs are made on an agar plate. A sample of bacteria is placed next to the discs, and after incubation, the zone of inhibition of bacterial growth around each disc is evaluated. The width of this zone indicates the susceptibility of the bacteria to a particular antibiotic.
5. Analysis of results: The obtained data on microflora and antibiotic sensitivity are analyzed by specialists. This helps to choose the most effective antibiotic for the treatment of a particular patient and prevents unnecessary use of antibiotics, which can lead to bacterial resistance.

An automated analyzer simplifies this process, accelerating results and reducing the likelihood of errors. It ensures the accuracy and reliability of data, which is important for the correct choice of treatment.

Bacteriological examination of nasal passages and determination of antibiotic sensitivity is an important stage in the diagnosis and treatment of various diseases, especially in the context of increasing bacterial resistance to antibiotics. It helps to select the optimal therapy regimen for each patient, ensuring more effective and safe treatment.